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Tissue chemistry

Jennifer Funk
Contributors : Jennifer.Funk262 points 


Protocols that receive sufficient votes and a high star rating will be considered for Gold Leaf Status by the PrometheusWiki Editorial Board.



Contributing Editor

Jennifer Funk

Summary

Plants contain a vast array of compounds and the isolation and quantification of these compounds is of interest to researchers in the fields of analytical chemistry, biochemistry, molecular biology, and plant physiology. Consequently, the tissue chemistry section contains a broad array of protocols for the analysis of elements and molecules in leaf tissue.

Inorganic compounds
Measuring the concentration of inorganic compounds such as nitrate, ammonium, and calcium can inform us about plant nutrition. However, many inorganic compounds, particularly metals, are also toxic to plants.

N and P
Some of the most basic assessments of plant function include measures of leaf nitrogen and phosphorus. Measures of biologically available N are important for studies of herbivory.

Pigments
Measures of various pigments including chlorophyll, carotenoids, and anthocyanin are useful in determining plant adaptation/acclimation to light and light stress.

Primary metabolites
We include several sub-sections for primary metabolites including carbohydrates, lipids, amino acids, nucleic acid, near infrared reflectance (NIR) spectroscopy, and proteins.

Secondary compounds
Currently our secondary metabolite sub-section is limited to phenolics, but additional sections on N-containing secondary compounds could be added.

Structural compounds
Currently this sub-section includes a protocol for cell wall mass and nitrogen, but other structural fractions, such as cellulose, will be added.


Contributors to this page: Admin36802 points  , Jennifer.Funk262 points  , Neil Moreton and 138.194.235.40 .
Page last modified on Friday 15 of October, 2010 08:33:57 EST by Admin36802 points . (Version 23)